This bacteria is usually considered a commensal, however it can cause significant infections rarely. In many species of bacteria, the quorum sensing mechanism is used as a unique communication system which allows them to regulate gene expression and behavior in accordance with their population density. Kluyvera strains ferment many of the sugars and polyhydroxyl alcohols used in identification. Kluyvera ascorbata sepsis in an extremely low birth weight. Draft genome sequence of kluyvera intestini strain gt16. Kluyvera, a new redefined genus in the family enterobacteriaceae. We isolated two qnra3 positive strains, one of klebsiella pneumoniae he96 and one of kluyvera ascorbata kas96, from the feces of an immunocompromised outpatient. Kluyvera is present in the environment as a freeliving organism, in water, soil. Year country % n esbl reference 1999 pakistan 35 200 zaman, pak armed forces med j, 1999 1999 china, japan, taiwan, singapore, philippine 20 2193 bell, diagn microbiol infect dis, 2002 1999 hong kong 11. Kluyvera species are described infrequently in association with clinically significant infections, and infections caused by these gram negative rods are especially rare in immunocompetent children. Carbapenemresistant enterobacteriaceae have been a global public health issue in recent years. In addition, there are no reports to verify the role of gut symbionts in the b. In silico analyses have shown that this event has occurred at least nine times.
The human vaginal bacterial biota and bacterial vaginosis. Nacylhomoserine lactones ahls are known as diffusible autoinducer molecules involved in this communication network. Blood cultures grew kluyvera ascorbata, and antibiotics were given. Infections caused by kluyvera species in humans juan c. A chromosomally encoded homologue in shewanella algae, qnra3, has been described. Kluyvera ascorbata is proposed as the type species for the genus. Kluyvera is a relatively newly described member of the enterobacteriaceae family that rarely causes infections in humans. Note the pink precipitate surrounding the individual colonies. An ultrasound showed dilated extrahepatic bile ducts.
Rhizobium inoculation has shown beneficial effects on nodulation, grain yield, and protein content of peas mckenzie et al. Kimbrough iii 0 1 2 0 our cases in which kluyvera strains caused infection in humans were identified by a retrospective analysis of clinical microbiology laboratory records at the texas tech university health sciences center and university med. Farmer jj 3rd, fanning gr, huntleycarter gp, holmes b, hickman fw, richard c, brenner dj 1981 kluyvera, a new redefined genus in the family enterobacteriaceae. Plant growth promoting rhizobacteriaan efficient tool for. Cultivationindependent molecular methods have provided new insights regarding bacterial diversity in. Antimicrobial agents active against kluyvera strains include thirdgeneration. Biological activity and colonization pattern of the. Kluyvera ascorbata 1 bzb2107 e4 morganella morganii 3 bzb2108 es porteus mirabilis 3 bzb2109 e6 p. Here, a carbapenemresistant kluyvera cryocrescens strain scw was isolated from hospital sewage, and was then subjected to wholegenome sequencing wgs. Abdominal infection, bacteraemia, kluyvera ascorbata. In order to assess the relationship between the genus kluyvera and other members of the family enterobacteriaceae, the 16s rrna genes of type strains of the recognized kluyvera species, kluyvera georgiana, kluyvera cochleae, kluyvera ascorbata and kluyvera cryocrescens, were sequenced.
By deoxyribonucleic aciddeoxyribonucleic acid hybridization, strains of kluyvera were divided into three groups. Ampc or cephalosporinases which exhibits a greater hydrolysis for cephalosporins in comparison to benzylpenicillin. C, and their varying behavior against carbenicillin disks of 100. After 24hours, this inoculated macconkey agar culture plate cultivated colonial growth of gramnegative, rodshaped, and facultatively anaerobic, kluyvera ascorbata bacteria. Nosocomial outbreak of kluyvera cryocrescens bacteremia. Separation of kluyvera and buttiauxella by biochemical and. Here, we report the complete genome sequence of the novel, nonsporeforming kluyvera intestini strain gt16, isolated from the stomach of a patient with gastric cancer.
This culture was used in a presentation involving the description of the steps taken when attempting to define a new taxonomic species. Characterization of a carbapenemresistant kluyvera. Kluyvera bacteria can cause opportunistic infections in immunocompromised patients references. Sesbenia, iaa production 3 pseudomonas fluorescens soybean cytokinin 33 azoarcus sp.
Kluyvera is a small, motile, gramnegative bacillus within the enterobacteriaceae family. A prompt identification of kluyvera species in clinical. It is gramnegative, rodshaped and motile with peritrichous flagella. Abdominal infection, bacteraemia, kluyvera ascorbata, pyelonephritis, sepsis. Resistance plasmids were completely circularized and further bioinformatics. Kluyvera ascorbata, leclercia adecarboxylata, morganella morganii, pantoea enterobacter agglomerans, plesiomonas shigelloides.
In the pediatric population, it is described in association with clinically significant infections ranging from urinary tract infections to sepsis with multiorgan failure. Kluyvera cryocrescens bacteremia case reports scielo. Kluyvera ascorbata belongs to enterobacteriaceae family and is a gram negative microorganism. Kluyvera ascorbata canola, tomato siderophores, 117 sud 165 iaa production azotobacter sp. This enterobacteria is a freeliving organism, found in water, soil, sewers. Rice nitrogen fixation 39 enterobacter cloacae rice iaa production 79 pseudomonas sp. Blood, csf, joint fluid, pleural fluid, peritoneal fluid, pericardial fluid, bone, or from a internal body or tissue site brain, heart, kidney, liver, lymph. The bacterial biota of the human vagina can have a profound impact on the health of women and their neonates.
Kluyvera ascorbata, the type species of the genus and the. Infections due to kluyvera species ascp check sample mb 104 article pdf available in american journal of clinical pathology 534. We experienced a case of urinary tract infection in a 3monthold child caused by kluyvera ascorbata. Snomed ct browser uk snomed ct clinical edition nhs data migration april 2020. A, the typical dry, lactosepositive escherichia coli growing on macconkey mac agar. Rhizobium inoculation of legume crops is commonly practiced in most countries, and the importance of using rhizobial inoculants specific to the legume being planted is well established. Although it was originally described as a benign colonizer and saprophyte, kluyvera has also been described as causing a wide array of serious and potentially lifethreatening infections and should not be discounted. Kluyvera ascorbata is a bacterium, the type species of its genus. Kluyvera ascorbata sud16526 is a spontaneous siderophoreoverproducing mutant of k. Microbiology atlas of enterobacteriaceae free medical.
A case of urinary tract infection caused by kluyvera ascorbata in an. A transhepatic cholangiogram revealed diffusely dilated intrahepatic and extrahepatic ducts and large, smooth, amorphous filling defects within the common bile duct. Changes in the vaginal microbiota have been associated with several adverse health outcomes including premature birth, pelvic inflammatory disease, and acquisition of hiv infection. A case of urinary tract infection and severe sepsis caused. Kluyvera ascorbata produces a betalactamase that results in an atypical susceptibility pattern, including lowlevel resistance to certain antibiotics.
Nosocomial outbreak of kluyvera cryocrescens bacteremia volume 23 issue 2 jungchung lin, chihsiang chen, l. Obstructive cholangitis secondary to mucus secreted by a. Our aim is to determine the clinical significance of k. It is part of the normal human flora of the respiratory tract and gastrointestinal tract. Enterobacter intermedius other names i atcc 33110 atcc 51609 kluyvera cochleae muller et al. Incorporation of different chromosomal blactxm related genes from different species of kluyvera has derived in different ctxm clusters. To develop a better understanding of the functioning of k. This betalactamase is thought to be a possible progenitor of some plasmidencoded ctxms. Kluyvera is a gram negative, facultatively anaerobic bacterial and motile genus from the family of enterobacteriaceae which have peritrichous flagella. This organism is usually resistant to most commonly used antibiotics used as first line in neonatal units.
Gut microbiota promotes host resistance to lowtemperature. High levels of colicin resistance in escherichia coli. This finding aimed to characterize the production of ahl of a bacterial. Although it causes infections infrequently, it is responsible for causing a wide range of infections including severe sepsis 1, 2. The plasmidborne quinolone resistance gene qnra1 is prevalent in multidrugresistant enterobacteriaceae. Metagenomic deep sequencing reveals association of. This bacillus which was initially thought to be a benign pathogen which could be localized in the respiratory tract, gastrointestinal system and urinary tract has been observed to cause to clinically severe disease in various anatomic localizations in the last 30 years. Keywords kluyvera ascorbata 4 urinary tract infection 4. Kluyvera is a gram negative bacillus and was defined by kluyver and van niel for the first time in 1936. Acute cholecystitis and bacteremia caused by khuyvera. Get a printable copy pdf file of the complete article 2. Infections that are caused by kluyvera bacteria have been previously reported in the medical literature. Kluyvera ascorbata sepsis in an extremely low birth weight infant. Infections caused by kluyvera species in humans pdf.
Kluyvera ascorbata organism kluyvera ascorbata hide descriptions. Author summary the physiological mechanisms by which insects survive at low temperatures have not yet been discovered. It possesses 5,350 predicted proteincoding genes encoding virulence factors and antibiotic resistance proteins. It is believed to be the source of genes encoding ctxmtype extended spectrum blactamases esbls and it has the ability to transfer these genes to other.
A plasmidborne shewanella algae gene, qnra3, and its. Dna hybridization techniques, grouped them in the genus kluyvera with two species, k. Farmer jj, fanning gr, huntleycarter gp, holmes b, hickman fw, richard c, et al. Natural antimicrobial susceptibility patterns of kluyvera. Kluyvera ascorbata is a gram negative microorganism belonging to the family enterobacteriaceae. A comparative phylogenetic analysis based on these 16s rrna gene sequences and those available for. Kluyvera definition of kluyvera by medical dictionary.
Pdf kluyvera is a relatively newly described member of the. This separation is supported by the following findings. Kluyvera ascorbata atcc 33433, klebsiella aerogenes kctc 2190, kluyvera cryocrescens nbrc 102467, acinetobacter pittii phea2. By combining transcriptomic and metabolomic approaches, we found that the presence of gut symbionts, especially k.